6.0
ValidityScore
Valid or Invalid?
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2020Rodents
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Fan Ding, Xia Li -
«We documented the mRNA expressions in the current study to validate the role of apigenin on the modulation of IDD markers. The expression levels of the control genes demonstrated by RT-qPCR are shown in Figure 4. The results showed the mRNA expressions of asporin (4-fold), syndecan-4 (5.2-fold), TNF-α (2-fold), TNF-R1 (3-fold), COX-2 (2.7-fold), and periostin (3.2 fold) were significantly increased (P<0.01) in the IDD-induced group compared to those in the sham control group. However, the enhanced levels of these genes were decreased in the apigenin-treated group, indicating that the drug initiated the restorative mechanism to return normal functioning (Figure 4).»
- Organism: Mouse / Rat (Rodents)
- Notable Magnitude of Effect.
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2020Non-human Cells
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Che-Hwon Park, Hye-Ja Lee, Hye-Won Yu, Ji-Hye Kim, Kyungmin Kim, Seon-Young Min, Suyeong Kim, Young-Jin Park -
«CONCLUSIONS: In this study, we evaluated the effect of apigenin on major mediators of inflammatory and allergic responses in RAW264.7 and RBL-2H3 cell. Apigenin (100 μM) effectively inhibited NO production and cytokine expression (IL-1β, IL6, COX-2, and iNOS) as well as the phosphorylation of ERK and JNK associated with MAPK signaling pathway in RAW264.7 cell. Apigenin (30 μM) also inhibited the expression of cytokines (TNF-α, IL-4, IL-5, IL-6, IL-13, and COX-2) and FcεRIα/γ as well as the phosphorylation of signaling molecules (Lyn, Syk, PLCγ1, ERK, and JNK) corresponding to allergic responses pathway in RBL-2H3 cell. Moreover, apigenin (20 μM) significantly induced gene or protein expression (filaggrin, loricrin, AQP3, HA, HAS-1, HAS-2, and HAS-3) in HaCaT cell of molecules that play an important role in the physical barrier and water retention properties of the skin. Further, it increased the expression of antimicrobial peptides (HBD-1, HBD-2, HBD-3, and LL-37) that play an important role in acting as chemical barriers of HaCaT cell. However, apigenin has a very low solubility in water. Therefore, it is necessary to develop delivery systems such as liposomes, polymeric micelles, nanosuspension in order to improve absorption and bioavailability in consideration of absorption, distribution, metabolism, and excretion (ADME). Although additional research is warranted, the results of the present study highlight apigenin as a potential candidate for alleviating immune-related diseases and AD.»
- Organism: In vitro
- Notable Magnitude of Effect.
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2019Non-human Cells
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Cho C, Choi S, Eyun SI, Han SJ, Jang D, Jeon J, Jeong SY, Kang LJ, Kim CS, Lee H, Nam J, Oh E, Park CH, Park E, Shin YS, Yang S -
«Abstract: Although Hif-2α is a master regulator of catabolic factor expression in osteoarthritis development, Hif-2α inhibitors remain undeveloped. The aim of this study was to determine whether Cirsium japonicum var. maackii (CJM) extract and one of its constituents, apigenin, could attenuate the Hif-2α-induced cartilage destruction implicated in osteoarthritis progression. In vitro and in vivo studies demonstrated that CJM reduced the IL-1β-, IL-6, IL-17- and TNF-α-induced up-regulation of MMP-3, MMP-13, ADAMTS4, ADAMTS5 and COX-2 and blocked osteoarthritis development in a destabilization of the medial meniscus mouse model. Activation of Hif-2α, which directly up-regulates MMP3, MMP13, ADAMTS4, IL-6 and COX-2 expression, is inhibited by CJM extract. Although cirsimarin, cirsimaritin and apigenin are components of CJM and can reduce inflammation, only apigenin effectively reduced Hif-2α expression and inhibited Hif-2α-induced MMP3, MMP13, ADAMTS4, IL-6 and COX-2 expression in articular chondrocytes. IL-1β induction of JNK phosphorylation and IκB degradation, representing a critical pathway for Hif-2α expression, was completely blocked by apigenin in a concentration-dependent manner. Collectively, these effects indicate that CJM and one of its most potent constituents, apigenin, can lead to the development of therapeutic agents for blocking osteoarthritis development as novel Hif-2α inhibitors.»
- Organism: In vitro
- Notable Magnitude of Effect.
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#Animals, Apigenin / pharmacology*, Arthritis, Articular / drug effects*, Articular / metabolism, Cartilage, Chanmi Cho, Chondrocytes / drug effects*, Chondrocytes / metabolism, Cirsium / chemistry*, Experimental / drug therapy*, Experimental / metabolism, Inbred C57BL, Interleukin-1beta / metabolism, Li-Jung Kang, MEDLINE, Male, Matrix Metalloproteinase 13 / metabolism, Matrix Metalloproteinase 3 / metabolism, Menisci, Mice, NCBI, NIH, NLM, National Center for Biotechnology Information, National Institutes of Health, National Library of Medicine, Non-U.S. Gov', Osteoarthritis / drug therapy*, Osteoarthritis / metabolism, PMC6652892, PubMed Abstract, Research Support, Siyoung Yang, Tibial / drug effects, Tibial / metabolism, Tumor Necrosis Factor-alpha / metabolism, Up-Regulation / drug effects, doi:10.1111/jcmm.14418, pmid:31148341more... -
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2019Human Cells
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Alexandra Ghițu, Andrea Bor, Anja Schwiebs, Camelia Oprean, Claudia Farcas, Codruta Soica, Corina Danciu, Cristina Adriana Dehelean, Florina Bojin, Heinfried H. Radeke, Ioana Zinuca Pavel, Istvan Zupko, Oana Duicu, Stefana Avram -
«The antiproliferative property of Api (apigenin) was determined by means of MTT (Sigma-Aldrich, Budapest, Hungary) assay against A375 human melanoma cell. Experiments were carried out as described previously [20]. Briefly, cell were seeded into 96-well plates (5000 cell/well) and treated with different concentration of Api (0.3–60.0 µM) under standard conditions (37 ◦C, 5% CO2). After 72 h of incubation, 5 mg/mL MTT solution was added and the microplates were incubated for an additional 4 h. The resulting formazan crystals were dissolved in dimethyl sulfoxide and the absorbance was determined at 545 nm with an ELISA reader (Awareness Technology, Palm City, FL, USA). Cisplatin, a clinically used anticancer agent was applied as a reference agent. Sigmoidal concentration–response curves were fitted to the determined results and IC50 values were calculated by means of GraphPad Prism (GraphPad Software, San Diego, CA, USA). »
- Organism: In vitro
- Notable Magnitude of Effect.
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ranked
added it
1 year ago
on Apr 26, 2021