«RESULTS: Treatment of iron-overloaded rats with curcuminresulted in marked decreases in iron accumulation within liver and spleen. Iron-overloaded rats had significant increases in malonyldialdehyde (MDA), a marker of lipid peroxidation and nitric oxide (NO) in liver and spleen when compared to control group. The effects of iron overload on lipid peroxidation and NO levels were significantly reduced by the intervention treatment with curcumin (P<0.05). Furthermore, the endogenous anti-oxidant activities/levels in liver and spleen were also significantly decreased in chronic iron overload and administration of curcumin restored the decrease in the hepatic and splenic antioxidant activities/levels.
«RESULTS: Levels of iron and the response to alterations in dietary iron availability vary dramatically in a strain-specific manner among mouse species.34 To determine whether curcumin affects systemic iron, we first assessed the response of C3H mice to alterations in levels of dietary iron. We chose this strain because it has been used extensively in the study of chemopreventive agents, including curcumin.2,35–39Mice were fed defined diet containing 5, 12, 50, and 1000 mg iron/kg diet beginning at 5 weeks of age. Basal AIN93M dietcontains 48.4 mg iron/kg diet. We therefore operationally defined 50 mg/kg as a “normal” irondiet. Levels of iron in our diet thus ranged from 10-fold lower to 20-fold higher than normal iron levels. Mice were killed after 6 months. There was no effect of these dietary ironconcentration on weight gain over this period (not shown).»